Journal: bioRxiv

Article Title: Parabrachial neuron types categorically encode thermoregulation variables during heat defense

doi: 10.1101/2020.06.11.138370

Figure Lengend Snippet: a-c , Changes in basal T core ( a ), basal physical activity ( b ), and basal energy expenditure ( c ) after blocking of POA-projected LPB Pdyn neurons (n = 6 each; 4-6 weeks post viral injection). d-g , Changes of energy expenditure ( d-e ), and physical activity ( f-g ) during warm challenge ( d, f ) or cold challenge ( e, g ), respectively, after blocking of POA-projected LPB Pdyn neurons (n = 6 each; 6-8 weeks post viral injection). (– 360 – 0 min) and (0 – 360 min) represent the averaged EE between t = (– 360 – 0 min) and t = (0 – 360 min), respectively. All data are shown as mean ± s.e.m. All the p-values are calculated based on ordinary two-way ANOVA with Bonferroni’s corrections (right panels in a-g ). *p ≤ 0.05; ns, not significant.

Article Snippet: Primary antibodies include chicken anti-GFP (Abcam, #ab13970, 1: 1000), rabbit anti-Neuropeptide S (Abcam, #ab18252, 1: 500), rabbit anti-cfos (Synaptic systems, #226003, 1: 10000), guinea pig anti-cfos (Synaptic systems, #226004, 1: 500), rat anti-RFP (Chromotek, #5F8, 1: 1000), rabbit anti-Glutamate (Sigma, #G6642-.2ML, 1: 1000; see ( - ) for validation.), rabbit anti-Dynorphin A (Phoenix Pharmaceuticals, #H-021-03, 1:500) and Guinea pig anti-ProDynorphin (GeneTex, GTX10280, 1: 500).

Techniques: Activity Assay, Blocking Assay, Injection

Journal: bioRxiv

Article Title: Hippocampal trauma memory processing conveying susceptibility to traumatic stress

doi: 10.1101/2024.01.08.574632

Figure Lengend Snippet: Hippocampal activity during fear memory encoding (marked by tdTomato expression), remote fear memory recall (marked by cFos expression), as well as parvalbumin (PV) interneuron density were assessed by immunohistochemistry. Arrows indicate tdTomato + cFos + double-positive cells. ( AB ). No group differences were observed in the size of the engram recruited during stress-enhanced fear learning ( C ). However, susceptible animals displayed a smaller population of ventral hippocampal CA1 neurons active during remote fear memory recall ( D ), without any group differences in neuronal reactivation rate ( E ). Additionally, susceptible animals showed a decrease in activity of PV + neurons in the ventral hippocampal CA1 specifically during remote memory recall ( F ), which was joined by an increase in local PV + density ( G ). Data represent medians with interquartile ranges. **: p < .01, *: p < .05, main effect of group, @: p < .05, group x subregion interaction, %: p < .01, group x axis interaction, #: p < .05, group x axis x subregion interaction.

Article Snippet: Incubation of the primary antibodies was performed overnight (guinea pig anti-cFos, 1:750, 226004, Synaptic Systems; rabbit anti-PV, 1:1000, ab11427, ITK; or rabbit anti-HDAC2, 3 µg/µL, AB_2533908, Thermo Fisher) in PBS-BT for 18 hours at RT.

Techniques: Activity Assay, Expressing, Immunohistochemistry

Journal: bioRxiv

Article Title: Hippocampal trauma memory processing conveying susceptibility to traumatic stress

doi: 10.1101/2024.01.08.574632

Figure Lengend Snippet: Hippocampal HDAC2 fluorescence in cells active during fear memory encoding (marked by tdTomato expression) and remote fear memory recall (marked by cFos expression) were assessed by immunohistochemistry ( AB ). Neurons involved in memory encoding (tdTomato + ) and recall (cFos + ), as well as reactivated (tdTomato + cFos + ; indicated by arrows) neurons, were characterized by overall higher HDAC2 fluorescence than non-engram cells. HDAC2 levels in the ventral hippocampus were modulated by a subregion x group interaction, which seemed to be caused by a tendency towards lower HDAC2 levels in the vCA1 in susceptible animals ( CDE ). Data represent medians with interquartile ranges. %: p < .001, main effect of axis, $: p < .001, main effect of subregion, &: p < .001, main effect of engram type, #: p < .05, group x axis x subregion interaction, @: p < .05, group x subregion interaction.

Article Snippet: Incubation of the primary antibodies was performed overnight (guinea pig anti-cFos, 1:750, 226004, Synaptic Systems; rabbit anti-PV, 1:1000, ab11427, ITK; or rabbit anti-HDAC2, 3 µg/µL, AB_2533908, Thermo Fisher) in PBS-BT for 18 hours at RT.

Techniques: Fluorescence, Expressing, Immunohistochemistry

Journal: bioRxiv

Article Title: Hippocampal trauma memory processing conveying susceptibility to traumatic stress

doi: 10.1101/2024.01.08.574632

Figure Lengend Snippet: Hippocampal 5mC and 5hmC fluorescence in cells active during fear memory encoding (marked by tdTomato expression), remote fear memory recall (marked by cFos expression) and both were assessed by immunohistochemistry and compared to non-engram cells (tdTomato and cFos negative cells) ( AB ). 5mC levels were higher in encoding and recall cells compared to non-engram cells. In contrast, reactivated cells displayed lower 5mC levels than non-engram cells. Importantly, susceptible mice displayed higher 5mC levels in memory encoding, recall, and non-engram cells, compared to resilient mice, without significant differences in reactivated cells ( C ). 5hmC levels were lower in all types of engram cells compared to non-engram cells, and susceptible mice displayed higher 5hmC levels in the ventral hippocampus ( D ). Data represent medians with interquartile ranges. $: p < .05, main effect of subregion, &: p < .001, main effect of engram type, *: p < .05, main effect of group, ¥: p < .05, group x engram type, %: p < .001, group x axis interaction.

Article Snippet: Incubation of the primary antibodies was performed overnight (guinea pig anti-cFos, 1:750, 226004, Synaptic Systems; rabbit anti-PV, 1:1000, ab11427, ITK; or rabbit anti-HDAC2, 3 µg/µL, AB_2533908, Thermo Fisher) in PBS-BT for 18 hours at RT.

Techniques: Fluorescence, Expressing, Immunohistochemistry

Journal: Stem cells (Dayton, Ohio)

Article Title: Retinoic Acid-signaling regulates the proliferative and neurogenic capacity of Müller glia-derived progenitor cells in the avian retina

doi: 10.1002/stem.2742

Figure Lengend Snippet: Antibodies, sources and working dilutions. Patterns of labeling and stimulus-dependent changes in levels of immunolabeling using these antibodies are consistent with previous reports (Fischer and Omar, 2005; Fischer et al., 2009a , 2009b ; Fischer et al., 2014 ; Todd and Fischer, 2015 ).

Article Snippet: Secondary antibodies included donkey-anti-goat-Alexa488/568, goat-anti-rabbit-Alexa488/568/647, goat-anti-mouse-Alexa488/568/647, goat anti-rat-Alexa488 (Life Technologies) diluted to 1:1000 in PBS plus 0.2% Triton X-100. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Antigen Working dilution Host Clone or catalog number Source Sox2 1:1000 goat Y-17 Santa Cruz Immunochemicals Sox9 1:2000 mouse AB5535 Chemicon Pax6 1:1000 rabbit PRB-278P Covance Klf4 1:50 rabbit {"type":"entrez-protein","attrs":{"text":"ARP38430","term_id":"1190169341","term_text":"ARP38430"}} ARP38430 Aviva Systems Biology cFos 1:400 rabbit K-25 Santa Cruz Immunochemicals Glutamine Synthetase 1:2000 mouse ab125724 Abcam CD45 1:200 Mouse HIS-C7 Cedi Diagnostic Nkx2.2 1:80 Mouse 74.5A5 DSHB CRABP 1:1000 Mouse C1 Dr. J Saari, University of Washington Lim 1/2 1:50 Mouse 4F2 DSHB Lim 3 1:100 Mouse 67.4E12 DSHB Otx2 1:1000 Goat AF1979 R&D Systems HuD/HuC 1:600 Mouse A21271 Invitrogen Open in a separate window Antibodies, sources and working dilutions.

Techniques: Labeling, Immunolabeling, Diagnostic Assay